The CRISPR-Cas9 system has proven to be a versatile tool for genome editing, with numerous implications in medicine, agriculture, bioenergy, food security, and beyond. The range of targetable DNA sequences is limited, however, by the need for a short sequence of DNA beside the target site, called the PAM. In total, there are only a handful of CRISPR enzymes with a short enough PAM sequence to be able to target a large portion of the total DNA in a genome. In this study, we identify a natural Cas9 enzyme from the bacterial genome of Streptococcus canis that has a PAM sequence with only a single G as its PAM sequence (5’-NNG-3’), allowing flexible targeting of up to 50% of all DNA sequences in living organisms. This new molecular tool potentially grants unprecedented access to correct disease-related mutations, enhance agricultural methods, and expand research efforts.